产品货号：
GS4570
中文名称：
重组小鼠CNTF
英文名称：
Recombinant Murine Ciliary Neurotrophic Factor
产品规格：
5μg
发货周期：
1～3天
产品价格：
询价
General Description
Recombinant murine CNTF containing 197 amino acids and it shares 82% and 95% a.a.sequence identity with human and rat CNTF.
Background
Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations.CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture.Furthermore,the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks.In addition,CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress.
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.
Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by Biorab for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
Molecular Weight
Approximately 22.5kDa,a single non-glycosylated polypeptide chain containing 197 amino acids.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30ng/ml,corresponding to a specific activity of> 3.3×104 IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 2×PBS,pH7.4,2% trehalose.
Endotoxin
Less than 1 EU/μg of rMuCNTF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.Further dilutions should be made in appropriate buffered solutions.
AA Sequence
AFAEQSPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH
QGLNKNISLD SVDGVPVAST DRWSEMTEAE
RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT
LTLQVSAFAY QLEELMALLE QKVPEKEADG MPVTIGDGGL
FEKKLWGLKV LQELSQWTVR SIHDLRVISS HHMGISAHES
HYGAKQM
相关搜索：重组小鼠CNTF，小鼠CNTF重组蛋白，Recombinant Murine Ciliary Neurotrophic Factor
Recombinant murine CNTF containing 197 amino acids and it shares 82% and 95% a.a.sequence identity with human and rat CNTF.
Background
Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations.CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture.Furthermore,the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks.In addition,CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress.
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.
Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by Biorab for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
Molecular Weight
Approximately 22.5kDa,a single non-glycosylated polypeptide chain containing 197 amino acids.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30ng/ml,corresponding to a specific activity of> 3.3×104 IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 2×PBS,pH7.4,2% trehalose.
Endotoxin
Less than 1 EU/μg of rMuCNTF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.Further dilutions should be made in appropriate buffered solutions.
AA Sequence
AFAEQSPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH
QGLNKNISLD SVDGVPVAST DRWSEMTEAE
RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT
LTLQVSAFAY QLEELMALLE QKVPEKEADG MPVTIGDGGL
FEKKLWGLKV LQELSQWTVR SIHDLRVISS HHMGISAHES
HYGAKQM
相关搜索：重组小鼠CNTF，小鼠CNTF重组蛋白，Recombinant Murine Ciliary Neurotrophic Factor